Speaker
X. Zhang
(TU München, Physik-Department, Physik weicher Materie)
Description
Polymer drug carriers based on HPMA copolymers have been studied extensively over the past few years [1,2], to understand the behavior and shape of the copolymers in dilute solutions. However, there is a lack of knowledge on the behavior of these drug carriers in real human blood environment.
We use HPMA copolymers labeled with fluorescent dyes, which are dissolved in a solution of human serum albumin (HSA). This facilitates the determination of their size by fluorescence life-time correlation spectroscopy (FLCS). Compared with FCS, a separate autocorrelation function is calculated in FLCS for each fluorescent component determined by its life-time, which enables an easier separation of different contributions.
[1] S. K. Filippov, et al., Biomacromolecules, 2012, 13 (8), 2594–2604
[1] S. K. Filippov, et al., Biomacromolecules, 2013, 14 (11), 4061–4070
Primary author
X. Zhang
(TU München, Physik-Department, Physik weicher Materie)
Co-authors
B.J. Niebuur
(TU München, Physik-Department, Physik weicher Materie)
Prof.
C. M. Papadakis
(TU München, Physik-Department, Physik weicher Materie)
E. Lomkova
(Institute of Macromolecular Chemistry, Academy of Sciences of the Czech Republic)
Dr
P. Chytil
(Institute of Macromolecular Chemistry, Academy of Sciences of the Czech Republic)
Dr
S. K. Filippov
(Institute of Macromolecular Chemistry, Academy of Sciences of the Czech Republic)
