Protein-Eye View of the in Meso Crystallization Mechanism

by Dr Leonie van't Hag (Monash University)

Monday 22 Jun 2020, 13:00 → 14:00 Europe/Berlin

Zoom Webinar

Understanding protein-lipid interactions is crucial for evolving food technology, biological and biomedical applications of nanomaterials. Knowledge regarding the effect of the multiple components in the system on the liquid crystal nanostructure, within the context of the application, is needed. Lyotropic liquid crystal design rules were developed and the effect of protein encapsulation on lipid self-assembly materials was extensively studied by us in recent years [1].

 

Recently, we made significant progress in this field by developing a small-angle neutron scattering method with perfectly contrast-matched bulk lipid phases in D2O to study biomolecule encapsulation [2]. In this presentation, I will show how we used this to obtain a protein-eye view of the in meso crystallisation method of integral membrane proteins from the bicontinuous cubic phase over time [3]. Studies to date have, by necessity, focused on structural transitions occurring in the lipid mesophase. This opens up the possibility of studying the structure (and function) of proteins, therapeutic peptides as well as other biomolecules in a lipid environment.

 

[1] L. van ‘t Hag, S.L. Gras, C.E. Conn, C.J. Drummond, Chem. Soc. Rev. 46, 2705-2731 (2017).
[2] L. van ‘t Hag, L. de Campo, C.J. Garvey et al., J. Phys. Chem. Lett., 7(14), 2862-2866 (2016).
[3] L. van ‘t Hag, L. de Campo, N. Tran et al, Langmuir, 35(25), 8344-8356 (2019).