Can mRNA - Lipid nanoparticle surface composition regulate Apolipoprotein binding from serum?

by Dr Federica Sebastiani

Monday 30 Nov 2020, 14:30 → 15:30 Europe/Berlin

Zoom Webinar

Lipid nanoparticles (LNPs) are promising vehicles for mRNA delivery and are formed by a cationic ionizable lipid (CIL), DSPC, cholesterol (Chol) and a pegylated (PEG) lipid. The bio-distribution and cellular uptake of LNPs are affected by their surface composition as well as by the extracellular proteins present at the site of LNPs administration, such as proteins in the plasma.

We employed small angle neutron scattering (SANS) to reveal the precise location of cholesterol and CIL across the LNPs. Additionally, we determined the extent of ApoE binding to LNP and subsequently the effect that protein binding exerts on the lipid distribution within the LNP particle.