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17–19 Sept 2018
Fakultät für Maschinenwesen der Technischen Universität München
Europe/Berlin timezone

Internal dynamics of different folding intermediates of apo-myoglobin

17 Sept 2018, 16:30
1h 30m
Fakultät für Maschinenwesen der Technischen Universität München

Fakultät für Maschinenwesen der Technischen Universität München

Boltzmannstraße 15 85748 Garching b. München
Poster P3 Structure and dynamics in life sciences Poster session 1

Speaker

Ms Livia Balacescu (Forschungszentrum Jülich GmbH, Jülich Centre for Neutron Science (JCNS) at Heinz Maier-Leibnitz Zentrum (MLZ))

Description

During protein folding different intermediate states occur with varying content of secondary structural elements. Regions lacking well-defined structure exist as well in unfolded and partially folded systems. In order to characterize their dynamics, we studied apo-myoglobin in different folding states [1,2,3]. We investigated internal dynamics of its unfolded form on a time-scale up to several hundred nanoseconds and in the nanometer length-scale using neutron spin echo spectroscopy (NSE) at the instrument SNS-NSE in Oak Ridge[4]. We measured the folded protein with NSE on the same time scales for comparison at the J-NSE “Phoenix” at MLZ. Aggregation state and center of mass diffusion were monitored in both cases in parallel with dynamic light scattering. Information on the form and structure factor was obtained by small angle neutron scattering (SANS) at KWS-2 at MLZ. Whereas the dynamics of the folded protein is dominated by center of mass diffusion, our first NSE data of the unfolded protein state shows a polymer-like behavior. This indicates that powerful polymer models well-established in the field of soft matter may be used to describe such protein systems.

[1] J. Phys. Chem. B, 2015, 119 (1), 72
[2] J Mol. Biol., 1996, 263(4), 531
[3] J. Am. Chem. Soc., 2014, 136 (19), 6987
[4] Richter D. et al., 2005, Neutron Spin Echo in Polymer Systems. Advances in Polymer Science, vol 174. Springer, Berlin, Heidelberg

Primary authors

Ms Livia Balacescu (Forschungszentrum Jülich GmbH, Jülich Centre for Neutron Science (JCNS) at Heinz Maier-Leibnitz Zentrum (MLZ)) Tobias Schrader Dr Andreas Stadler (JCNS1 & ICS3 FZ Jülich) Prof. Jörg Fitter (I. Physikalisches Institut RWTH Aachen)

Co-authors

Aurel Radulescu (Jülich Centre for Neutron Science - Outstation at MLZ) Dr Piotr Zolnierczuk (Forschungszentrum Jülich GmbH, Jülich Centre for Neutron Science (JCNS) at the SNS Oak Ridge National Laboratory) Olaf Holderer

Presentation materials

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