Neutrons for Science and Industry

Can mRNA - Lipid nanoparticle surface composition regulate Apolipoprotein binding from serum?

by Dr Federica Sebastiani

Europe/Berlin
Zoom Webinar

Zoom Webinar

https://tum-conf.zoom.us/j/95452182512 Passwort: 912797
Description

Lipid nanoparticles (LNPs) are promising vehicles for mRNA delivery and are formed by a cationic ionizable lipid (CIL), DSPC, cholesterol (Chol) and a pegylated (PEG) lipid. The bio-distribution and cellular uptake of LNPs are affected by their surface composition as well as by the extracellular proteins present at the site of LNPs administration, such as proteins in the plasma.

We employed small angle neutron scattering (SANS) to reveal the precise location of cholesterol and CIL across the LNPs. Additionally, we determined the extent of ApoE binding to LNP and subsequently the effect that protein binding exerts on the lipid distribution within the LNP particle. 

Organized by

Dr. Christian Franz
Dr. Christian Lang

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