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14–17 Mar 2022
Europe/Berlin timezone

Identifying metal redox states through low dose measurements for spatially resolved anomalous dispersion refinement

15 Mar 2022, 09:40
20m
Talk Biologic Structure, Function, Reactivity, and Regulation Methods in Biocrystallography

Speaker

Dr Frank Lennartz (Macromolecular Crystallography, Helmholtz-Zentrum Berlin für Materialien und Energie (HZB-MX))

Description

Here, we describe the application of Spatially Resolved Anomalous Dispersion (SpReAD) refinement as a method to determine the oxidation state of protein-bound, small metal co-factors through x-ray crystallography, and show the influence of total absorbed dose on these experiments.

Primary authors

Dr Frank Lennartz (Macromolecular Crystallography, Helmholtz-Zentrum Berlin für Materialien und Energie (HZB-MX)) Prof. Holger Dobbek (Humboldt-Universität zu Berlin) Dr Jae-Hun Jeoung (Humboldt-Universität zu Berlin) Dr Manfred Weiss (Macromolecular Crystallography, Helmholtz-Zentrum Berlin für Materialien und Energie (HZB-MX)) Mr Stefan Rünger (Humboldt-Universität zu Berlin)

Presentation materials