Speaker
Wulf Blankenfeldt
(DGK)
Description
PlaB is a secreted phospholipase of Legionella pneumophila, the causative agent of Legionnaires' Disease. It is an unusual enzyme that looses activity at higher concentrations due to tetramer formation.
Here, we show the crystal structure of PlaB in its inactive tetrameric form. We find that the tetramer is stabilized by NAD(H), a central metabolite only found within the cell. This ligand-mediated oligomerization may hence establish a self-protection mechanism.
Primary authors
Prof.
Antje Flieger
(Robert Koch Institute, Wernigerode)
Dr
Maurice Diwo
(Helmholtz Centre for Infection Research)
Wulf Blankenfeldt
(DGK)
Dr
Wiebke Michel
(Robert Koch Institute, Wernigerode)
