Speaker
Description
Single chain nano particles (SCNPs) are model system to decipher intrinsically disordered proteins. Here, we have probed the effect of salt/polymer concentration, cross link density, and charge density. Poly-l-lysine-SCNPs (PLL-SCNPs) prepared by tuning intramolecular crosslinking, solvent condition and ionic strength. Structural result show that radius of gyration of PLL-SCNP lowers by varying the crosslinker and salt concentration compared to linear PLL (PLL). PLL, PLL-SCNP with RNA, PLL/RNA and PLL-SCNP/RNA respectively, and acetylated PLL (ac-PLL/RNA) form polyelectrolyte complex (PEC) coacervate. The correlation length reduces for PLL-SCNP/RNA suggest variation in the internal topology. Topology probed independent of charge density was ensured by reacting ac with PLL which show similar correlation length as PLL-SCNP/RNA. Furthermore, we have studied the effect of nonsteroidal anti-inflammatory drugs (NSAIDs), aspirin (Asp), diclofenac (Diclo) and ibuprofen (Ibu) on the dynamics of brain lipids (BLs) unilamellar vesicles (BLs-ULVs), physiologically relevant membrane, using quasi elastic neutron scattering (QENS) and neutron spin echo (NSE). It is found that Asp, Diclo and Ibu fasten whole lipid diffusion at highest, lowest and intermediate concentration, respectively. NSE result show that, Asp stiffens, Diclo softens BLs membrane and Ibu does not affect bending rigidity (κKBT). This study enhances our understanding of the biomimetic systems and its biomedical implications.
