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May 22 – 25, 2023
Europe/Berlin timezone

Berberine loaded liposome system structure investigated by SANS and SAXS

May 23, 2023, 5:20 PM
1h 10m
Schloss Fürstenried (Munich)

Schloss Fürstenried


Forst-Kasten-Allee 103 81475 München Germany
Poster Lipids and membranes Poster session


Mrs Sabina Emanuela Erhan (Babeș-Bolyai University, Faculty of Biology and Geology, Doctoral School of Integrative Biology, Cluj-Napoca, RO)


In the recent years, plant extract became more and more used, worldwide in allopathic treatment of different types of disease associated symptoms. Several studies showed that plant extracts containing a certain alkaloid, known as berberine, has different beneficial effects such as antitumoral effect (Andreicuț et all, 2019; Milata et all, 2019). Other effect of these berberine based plant extract, are antioxidant and anti-inflammatory (Andreicuț et all, 2018).
Liposomes are concentrical bistratified vesicles, in which the liquid phase is fully locked in a double lipidic layer, mainly made of synthetic or natural phospholipids. One liposome can be synthesized in different forms, such as unilamellar and multilamellar vesicles (Shukla et. All, 2018).
Since berberine is an instable alkaloid (Duong et. all, 2021) and vegetal extracts of interest for our study (Berberis vulgaris, Mahonia aquifolium and Phellodendron amurese) have a considerable amount of berberine, we can assume that enclosing them into liposomes would offer them a more chemical stabile form, compared with the use of these extracts as they are (in a freeway). The vegetal extracts were obtained through cold percolation method (Pârvu et. all, 2013), with plant parts collected from „Alexandru Borza” Bothanical Garden, Babeș-Bolyai University, Cluj-Napoca, Romania.
For obtaining more information about our systems, such as form and structure, we have considered using small angle X-ray and neutron scattering (SAXS and SANS) methods. Our systems were composed from unilamellar vesicles (ULV), multilamellar vesicles (MLV) and our vegetal extracts of interest: Berberis vulgaris, Mahonia aquifolium and Phellodendron amurese. The vesicles were obtained from Dimyristoylphosphatidylcholine – DMPC. SANS measurements were performed at the YuMO neutron spectrometer at the IBR-2 reactor, Frank Laboratory of Neutron Physics and SAXS measurements were performed at BioSAXS instrument, BM29 beamline, European Synchrotron Radiation Facility.
From the obtained data we can say that our systems have demonstrated a stabilizing effect of the vegetal extracts, but in the case of Mahonia aquifolium this effect was more visible. We can also say that tempeature does not influence the stability or the structure of the systems. Also with the obtained data we can make theoretical models for our systems, to see their form and structure more clear.

Andreicuț, A.D., Pârvu, A.E., Moț, A.C., Pârvu, M., Fischer-Fodor, E., Cătoi, A.F., Feldrihan, V., Cecan, M., Irimie, A., Phytochemical Analysis of Anti-inflammatory and antioxidant effects of Mahonia aquifolium flower and fruit extracts, Hindawi, Oxidative medicine and cellular longevity, 2018, article ID 2879793, 12 pp., https://doi.org/10.1155/2018/2879793
Andreicuț, A.D., Fischer-Fodor, E., Pârvu, A.E., Țigu, A.B., Cenariu, M., Pârvu, M., Cătoi, F.A., Irimie, A., Antitumoral and immunomodulatory effect of Mahonia aquifolium extracts, Hindawi, Oxidative medicine and cellular longevity, 2019, article ID 6439021, 13 pp., https://doi.org/10.1155/2019/6439021
Milata, V., Svedova, A., Berbierikova, Z., Holubkova, E., Cipakova, I., Cholujova, D., Jakubikova, J., Panik, M., Jantova, S., Brezova, V., Cipak, L., Synthesis and anticancer activity of novel 9-O-substituted berberine derivatives, Int. J. Mol. Sci., 2019, 20:2169, 18pg., doi:10.3390/ijms20092169
Pârvu, M., Vlase, L., Fodorpataki, L., Pârvu, O., Bartha, C., Roșca-Casian, O., Barbu-Tudoran, L., Pârvu, A.E., Chemical composition of celandine (Chelidonium majus L.) extract and its effects on Botrytis tulipae (Lib.) lind fungus and the tulip, Not. Bot. Hort. Agrobot., 2013, 41 (2), 414–426, http://doi.org/10.15835/nbha4129077
Shukla, T., Upmanyu, N., Pandey, S.P., Gosh, D., Chapter 1 – Lipid nanocarriers, Lipid nanocarriers for drug targeting, 2018, pp. 1-47, https://doi.org/10.1016/B978-0-12-813687-4.00001-3

Primary author

Mrs Sabina Emanuela Erhan (Babeș-Bolyai University, Faculty of Biology and Geology, Doctoral School of Integrative Biology, Cluj-Napoca, RO)


Dr Augustin Cătălin Moț (Faculty of Chemistry and Chemical Engineering, Babeș-Bolyai University, Cluj-Napoca, RO) Prof. Marcel Pârvu (Babeș-Bolyai University, Faculty of Biology and Geology, Cluj-Napoca, RO) Dr Raul Victor Erhan (Horia Hulubei National Institute for R&D in Physics and Nuclear Engineering IFIN - HH, RO)

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