Dec 8 – 10, 2020 Online only
Online event
Europe/Berlin timezone

Inorganic nanoparticles challenging lamellar and non-lamellar lipid membranes: the role of curvature in nano-bio interactions

Dec 8, 2020, 3:00 PM
25m
Online event

Online event

Invited talk UM: Soft Matter MLZ Users 2020 - Soft Matter

Speaker

Ms Lucrezia Caselli (University of Florence)

Description

The interaction of engineered nanomaterials with living systems is mediated by biological barriers, determining their biological fate and cytotoxicity. Understanding the interaction of nanoparticles (NPs) with biological interfaces is the key to fill the gap between NPs development and end-use application. Lipid-based synthetic membranes can be used to mimic natural interfaces under simplified conditions, in order to identify key determinants in nano-bio interactions. While most of investigations so far focused on lamellar models, far less attention is given to curved-bilayer structures, ubiquitous in cells under certain conditions. Here, we address the interaction of inorganic NPs with biomimetic bilayers of lamellar and non-lamellar nature, i.e. flat membranes to cubic architectures encountered in diseased cells. With a library of gold NPs, we explore the effect of NPs shape and surface coating as a function of membrane curvature. Through an ensemble of structural techniques (SAXS, GISANS and Neutron Reflectivity), we found that highly curved membranes are associated with an enhanced structural stability towards NPs. Moreover, Confocal Laser Scanning Microscopy analysis highlights that cubic and lamellar phases interact with NPs according to two distinct mechanisms. These results are the first attempt to systematically study the role of membrane curvature in the interaction with NPs, disclosing new perspectives on the understanding and application of Nano-bio Interfaces.

Primary author

Ms Lucrezia Caselli (University of Florence)

Co-authors

Mr Andrea Ridolfi (National Research Council, Institute of Nanostructured Materials (CNR-ISMN); University of Florence) Dr Costanza Montis (University of Florence) Jean-Francois Moulin (HZG) Gaetano Mangiapia (German Engineering Materials Science Centre (GEMS) am Heinz Maier-Leibnitz Zentrum (MLZ)) Tommy Nylander (Lund University) Prof. Debora Berti (University of Florence)

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