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8–9 Dec 2022
Marriott
Europe/Berlin timezone

Quantum cascade laser-based infrared spectrometer combined with small angle neutron scattering for life science applications.

8 Dec 2022, 17:35
25m
Marriott

Marriott

Talk (20 min + 5 min discussion) Soft Matter Soft Matter

Speaker

Dr Seyed Mohammad Mahdi Dadfar

Description

Using the amide I band, infrared (IR-) spectroscopy can give information on the fold of the protein and also allows to follow aggregation phenomena. Small angle neutron scattering also reports on the global structure of proteins in solution and can give information on the shape of growing aggregates or folded proteins in solution.
We would like to explore the capabilities of infrared spectroscopy based on quantum cascade lasers (QCLs) in combination with small angle neutron scattering (SANS). The advantages of QCLs are superior Gaussian beam characteristics and a higher spectral density as compared to the glow bar infrared light sources of the Fourier-transform infrared spectrometer (FTIR). Their disadvantage is the more complicated pulsed mode of operation and the limited spectral width they can cover.
As a first scientific sample, the effect of a pH drop on protein aggregation and amyloid like structure formation in insulin is investigated. Insulin was dissolved in a phosphate buffer, where the pH was adjusted to 2. At room temperature, the sample was pumped through varying combinations of flow through cells of the FTIR spectrometer, the QCL, the UV-Visible spectrophotometer and the static light scattering device. Thereby we could follow the amyloid like structure formation on the very same sample using many different techniques in parallel. In November we have a scheduled beamtime at SANS-1 at PSI to finally add SANS as one of those techniques.

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