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Dec 8 – 9, 2022
Marriott
Europe/Berlin timezone

The Macromolecular Neutron Single Crystal Diffractometer BIODIFF for Proteins at the Heinz Maier-Leibnitz Zentrum

Dec 9, 2022, 2:00 PM
3h
Marriott

Marriott

Board: P-099
Poster Structure Research Poster Session

Speaker

Andreas Ostermann (Heinz Maier-Leibnitz Zentrum (MLZ), Technische Universität München)

Description

Neutron single crystal diffraction provides an experimental method for the direct location of hydrogen and deuterium atoms in biological macromolecules. At the FRM II neutron source the neutron single crystal diffractometer BIODIFF, a joint project of the Forschungszentrum Jülich and the FRM II, is dedicated to the structure determination of enzymes. Typical scientific questions address the determination of protonation states of amino acid side chains, the orientation of individual water molecules and the characterization of the hydrogen bonding network between the enzyme active center and an inhibitor or substrate. This knowledge is often crucial towards understanding the specific function and behavior of an enzyme. BIODIFF is designed as a monochromatic diffractometer and is able to operate in the wavelength range of 2.4 Å to about 5.6 Å. This allows to adapt the wavelength to the size of the unit cell of the sample crystal. Data collection at cryogenic temperatures is possible, allowing studies of cryo-trapped enzymatic intermediates. Recently a hexapod has been installed at BIODIFF which allows an online collimator alignment. Some recent examples will be presented to illustrate the potential of neutron macromolecular crystallography. In addition, a potential detector upgrade will be presented.

Primary authors

Andreas Ostermann (Heinz Maier-Leibnitz Zentrum (MLZ), Technische Universität München) Tobias Erich Schrader (Forschungszentrum Jülich GmbH, Jülich Centre for Neutron Science (JCNS) at Heinz Maier-Leibnitz Zentrum (MLZ), Garching, Germany)

Presentation materials

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