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20–23 Mar 2023
Campus Garching
Europe/Berlin timezone

How Polymorphism and Ligand Binding modulate G-quadruplex Fast Dynamics

20 Mar 2023, 16:00
2h
Yards 4 - 6 (Fakultät für Maschinenwesen)

Yards 4 - 6

Fakultät für Maschinenwesen

Board: MO-025
Poster Health and Life Sciences Poster Session MONDAY

Speaker

Luca Bertini (Università degli Studi di Perugia)

Description

G-quadruplexes (G4s) formed by the human telomeric sequence AG3(TTAG3)3 (Tel22) play a key role in cancer and ageing. G4 structures are known to display a variety of topologies, which are determined by several factors, resulting in structural polymorphism. Neutron Scattering techniques are a valuable tool to investigate how G4 structural polymorphism and ligand binding affect their sub-nanosecond dynamics. Within this context, we combined FTIR spectroscopy to monitor the Tel22 conformation and EINS to assess the corresponding dynamical properties. K+ and Na+ stabilized G4s were found to be in the parallel and mixed parallel-antiparallel topologies, respectively, with the latter resulting to be dynamically more stable. This result is compatible with the presence of ordered hydration-water structures in the antiparallel conformation. Complexation with the model ligand BRACO19 (BR19) resulted in an overall increase of Tel22 mobility. Such a dynamical enhancement, which is uncorrelated to the G4 topology, can be ascribed to a preferential binding of water molecules to Tel22 rather than to BR19.

Primary authors

Prof. Alessandro Paciaroni Luca Bertini (Università degli Studi di Perugia)

Co-authors

Prof. Andrea Orecchini (Università degli Studi di Perugia) Prof. Caterina Petrillo (Università degli Studi di Perugia) Francesca Natali (CNR-IOM) Dr Francesca Ripanti (Università degli Studi di Perugia) Dr Lucia Comez (Istituto Officina dei Materiali (IOM-CNR)) Prof. Marco Paolantoni (Università degli Studi di Perugia) Dr Valeria Libera (Università degli Studi di Perugia)

Presentation materials

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