Mar 20 – 23, 2023
Campus Garching
Europe/Berlin timezone

How Polymorphism and Ligand Binding modulate G-quadruplex Fast Dynamics

Mar 20, 2023, 4:00 PM
Yards 4 - 6 (Fakultät für Maschinenwesen)

Yards 4 - 6

Fakultät für Maschinenwesen

Board: MO-025
Poster Health and Life Sciences Poster Session MONDAY


Luca Bertini (Università degli Studi di Perugia)


G-quadruplexes (G4s) formed by the human telomeric sequence AG3(TTAG3)3 (Tel22) play a key role in cancer and ageing. G4 structures are known to display a variety of topologies, which are determined by several factors, resulting in structural polymorphism. Neutron Scattering techniques are a valuable tool to investigate how G4 structural polymorphism and ligand binding affect their sub-nanosecond dynamics. Within this context, we combined FTIR spectroscopy to monitor the Tel22 conformation and EINS to assess the corresponding dynamical properties. K+ and Na+ stabilized G4s were found to be in the parallel and mixed parallel-antiparallel topologies, respectively, with the latter resulting to be dynamically more stable. This result is compatible with the presence of ordered hydration-water structures in the antiparallel conformation. Complexation with the model ligand BRACO19 (BR19) resulted in an overall increase of Tel22 mobility. Such a dynamical enhancement, which is uncorrelated to the G4 topology, can be ascribed to a preferential binding of water molecules to Tel22 rather than to BR19.

Primary authors

Prof. Alessandro Paciaroni Luca Bertini (Università degli Studi di Perugia)


Prof. Andrea Orecchini (Università degli Studi di Perugia) Prof. Caterina Petrillo (Università degli Studi di Perugia) Francesca Natali (CNR-IOM) Dr Francesca Ripanti (Università degli Studi di Perugia) Dr Lucia Comez (Istituto Officina dei Materiali (IOM-CNR)) Prof. Marco Paolantoni (Università degli Studi di Perugia) Dr Valeria Libera (Università degli Studi di Perugia)

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