Mar 20 – 23, 2023
Campus Garching
Europe/Berlin timezone

Extracellular vesicles internalization mechanisms into cells

Mar 21, 2023, 4:00 PM
Yards 4 - 6 (Fakultät für Maschinenwesen)

Yards 4 - 6

Fakultät für Maschinenwesen

Board: TU-206
Poster Health and Life Sciences Poster session TUESDAY


Valeria Rondelli (Università degli Studi di Milano)


Extracellular vesicles (EV) are a potent intercellular communication system, delivering proteins and genetic material throughout the body, strongly influencing the fate of recipient cells. Due to their specific biological functions and to their peculiar molecular content they have been proposed as biomarkers for various diseases and as optimal candidates for therapeutic applications. Despite of their extreme biological relevance, their mechanisms of internalization into recipient cells are still hotly debated. We performed a multiscale investigation based on atomic force microscopy, small angle X-ray and neutron scattering, neutron reflectometry, infrared spectroscopy and scanning calorimetry to reveal the structural features of EV of different origin an to investigate their interaction with model membrane systems of variably complex composition, to spot the role of different membrane phases on the vesicles internalization routes. Our analysis reveals dependence of interaction mechanisms on EV nature. Our approach has clear implications on the modulation of EV internalization routes by targeting specific domains at the plasma cell membrane and, as a consequence, on EV-based therapies.

[F. Perissinotto & V. Rondelli et al., Nanoscale, 2021]
[M. Grava et al., Biomolecular Concepts, 2022]
[V. Rondelli et al., ACS Omega, 2022]
[M. Grava et al., Il Nuovo Cimento C, 2022]

Primary authors

Sally Helmy (Università degli Studi di Milano) Miriam Grava (Darmstadt University) Pietro Parisse (CNR-IOM Trieste) László Bottyán (BNC, Budapest) Daniel Merkel (BNC, Budapest) Stephen Hall (ISIS) László Almasy (BNC Budapest) Alexandros Koutsioumpas (JCNS) Paola Brocca (Università degli Studi di Milano) Loredana Casalis (ELETTRA sincrotrone Trieste) Valeria Rondelli (Università degli Studi di Milano)

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