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20–23 Mar 2023
Campus Garching
Europe/Berlin timezone

Influence of polarisation analysis on buffer subraction

20 Mar 2023, 12:10
20m
MW 2 001 - Lecture hall (Fakultät für Maschinenwesen)

MW 2 001 - Lecture hall

Fakultät für Maschinenwesen

Speaker

Dr Mona Sarter (SIS Pulsed Neutron and Muon Facility, Science and Technology Facilities Council)

Description

Protein dynamics play a vital role in biology. Quasi elastic neutron scattering is an ideal method to access these dynamics. Normally data analysis is performed based on the assumption that the scattering spectrum is incoherent. In order to be observe the full range of protein dynamics it is necessary to perform the experiments in solution. This solution is usually a fully deuterated buffer, while the protein remains protonated. It is generally assumed that while the buffer leads to a coherent contribution, this can be taken into account during data analysis by subtracting the buffer contribution from the sample spectrum. Up until recently there was no way to experimentally verify this assumption. Polarised QENS experiments on LET allow for the coherent and incoherent contributions to be separated. By comparing the results from the polarised QENS experiment and the standard analysis method from unpolarised QENS it was possible to experimentally check this assumption. The results and the implications for data treatment in the future will be discussed.

Primary author

Dr Mona Sarter (SIS Pulsed Neutron and Muon Facility, Science and Technology Facilities Council)

Co-authors

Ross Stewart (ISIS Neutron and Muon Source, UK) Kirill Nemkovskiy (ISIS Neutron and Muon Source, UK) Gøran Nilsen (ISIS Neutron and Muon Source)

Presentation materials

There are no materials yet.